Synthesis and biological profiling of novel isocoumarin derivatives and related compounds

In the continuation of our study substituted isocoumarins a series novel 3-azolyl isocoumarin and their thio derivatives, including some related lactone compounds was prepared biologically profiled against C. albicans showing moderate activity with MIC values in range 4?60 ?g mL-1, general. The additional characterisation selected carried out by exploring on CYP3A4 CYP2D6 enzymes, while experiments mutagenicity were performed AMES test. representative 3b, 4a 4b showed lower inhibitory CYP when compared to reference inhibitors, ketoconazole quinidine. Compound higher mutagenic potential than other two compounds. Further characterization included cytotoxicity profiling normal MRC5 cells.